Aseries figure2

This figure taken from


How was the neutrophil chemotaxis assay conducted?

Chemotaxis Assay.Edit

"Chemotaxis was performed in a 48-well microchamber (Neuro Probe) using a 5-μm-pore polycarbonate membrane. Neutrophils (1 × 106 cells/mL) were allowed to migrate toward CXCL2 (30 ng/mL) or medium alone at 37 °C with 5% CO2. After 1 h, the membrane was removed, fixed, and stained. Neutrophils that migrated through the membrane were counted under a light microscope on at least 5 randomly selected fields." From Alves-Filho et al. (2009)

A very detailed protocol to set up the plate and apply the samples can be found at

Suggested ReadingEdit

Falk, Goodwin, and Leonard. "A 48 Well Micro Chemotaxis Assembly for Rapid and Accurate Measurement of Leukocyte Migration." 1980, Journal of Immunological Methods, 33, 239-247.

Harvath, Falk, and Leonard. "Rapid Quantification of Neutrophil Chemotaxis: Use of a Polyvinylpyrrolidone-free Polycarbonate Membrane in a Multiwell Assembly." 1980, Journal of Immunological Methods, 37, 39-45.

Richards & McCullough A modified microchamber method for chemotaxis and chemokinesis." Immunological communications, Vol. 13, No. 1. (1984), pp. 49-62.

Harvath and Leonard. "Two Neutrophil Populations in Human Blood with Different Chemotactic Activities: Separation and Chemoattractant Binding." 1982, Infection and Immunity, 36 (2), 443-449.